r/bonecollecting Sep 12 '20

Advice Processing a Carcass 101 - the bones of bone collecting

1.2k Upvotes

Ok, so given how many comments we get requesting info on how to process a carcass, I figured it was time to update the stickie for this topic. Enjoy and, as always, feel free to comment and I will append this as needed. Just a reminder to A) always check your local and federal laws to make sure it is legal for you to possess parts of the animal, and B) if you are in an area prone to rabies or other diseases (rabbits and tularemia, armadillos and leprosy, etc), please take adequate precautions when handling dead animals, especially fresh carcasses. Always use gloves when handling a fresh carcass.

HOW TO PROCESS A CARCASS

There are generally three steps in the process of rendering a carcass down to a skeleton: 1) defleshing, 2) degreasing, 3) whitening. In general, these three steps are most effective when done sequentially. Two main things to remember during the process – Chlorine bleach should NEVER be used in any step of this process, and cooking bare bones will fix the grease and potentially cause long-term damage to the bones. Below are a few good guides for processing a carcass for you to take a look at.

http://www.jakes-bones.com/p/how-to-clean-animal-bones.html

http://baccyflap.com/txt/natmat/bones/

http://www.nara.accu.or.jp/img/elearning/2011/animal.pdf

http://bone-lust.blogspot.com/2015/05/bonelust-q-ive-been-macerating-bones.html

DEFLESHING

In general, the more flesh and skin that is removed, the faster the defleshing step is and the less smell you will have. Once you finish defleshing, you will want to pick clean any remaining soft tissue with tweezers, a scalpel, brush, etc before moving on to the degreasing step.

Open Air - This is easily the fastest method for defleshing. Using this method, you let the carcass rot naturally on the ground and let the flies and other insects work their magic. To do this technique, it is highly recommended that you use a locking cage to keep out scavengers that will be drawn to it. You simply put the carcass in the cage or fenced in area. You do not need to deflesh, skin, or gut the animal first for this to work, in fact the skin can help keep the moisture in. Sometimes it helps to poke a few extra holes for the maggots to get in. There are a few major drawbacks to this technique 1) the smell is awful and your neighbors will hate you, 2) you have to keep the carcass moist for the maggots to keep working, or you will end up with a mummified carcass, and 3) you will develop a fly problem. So, this is better done in a more warmer and more humid climate (doesn't work so well in a desert), and you only want to do this if you have adequate land and distance from your residence (and neighbors, think about your neighbors). Once the skeleton is reasonably clean, remove the bones and rinse them off.

Dermestids – great method if you have the ability to sustain a colony, and works well in the winter if you have a heated set-up. Rather than go through this process, here is a great link that goes over it. Be forewarned, dermestids will smell and do require you to keep feeding it as they are living creatures. If you do not properly ventilate, clean, or feed them, they will find a way to swarm out of their enclosure and I speak from personal experience when I say that you don’t want that to happen. Note that this is the only one of the defleshing techniques that will keep fish, birds, lizards, and small mammal skeletons somewhat intact. The other techniques mentioned below will result in disarticulation.

https://www.natsca.org/sites/default/files/publications/JoNSC-Vol7-Munoz-Saba_et_al_2020_0.pdf

Burying – this technique works best when you have a piece of property to do it on, have time, and can reasonably protect the carcass from scavengers. It also is the easiest for cleanup and has the least smell, and is a great method for when you are dealing with a whole carcass from a larger animal. This method also works with smaller animals, like rodents, if done in a flowerpot. You will still want to skin and deflesh as much as possible beforehand, and you’ll want to keep the soil slightly moist. With burying, there are two primary concerns: scavengers and loosing parts. To prevent scavenging, try to bury at least 2 ft (60 cm) deep (or deeper if sandy soils) and place larger rocks above the carcass to act as a barrier to digging. To prevent the loss of smaller elements, consider placing a wire mesh below the skeleton. The time it takes to decompose depends a lot on the local soil conditions (soil type, soil temperature, soil moisture, soil acidity), but will generally be several months for a larger carcass. I have heard of people adding bacteria (yeast) or compost to the carcass to help speed the process along. Oh, and one last helpful tip…place a clear marker over the pit so you can find it again when it is time.

Maceration – the smelliest method, but highly effective and you can use the same container the entire way through the process. You will want a large container with water, and a way to keep the water on the warmer side (over 70 F/21 C). If you can stomach it, stirring the pot every day will help with the maceration process. You will want to do pour-off’s (replacing the water) regularly initially as the water becomes too fouled (and to remove chunks of soft tissue that will invariable float around), and this is where the most offensive, gag-inducing, eye-watering, curse-laden part of the process will occur. But as the decomp gets farther along, do fewer pour offs. With each pour off, you are reducing the amount of bacteria for digesting the soft tissue. As long as there is plenty of food available, they will repopulate (try to leave some of the scum with each pour off to allow faster recovery of the bacterial population). If the water is allowed to get too cold, the decomposition process will stop and, even worse, you will convert the fats to adipocere (bone wax) which is very difficult to remove. Under ideal conditions, you can easily render a fleshed animal to bones in a few weeks using this method. You don’t necessarily need to deflesh for this technique to work (and I have found that having the bacteria from the stomach contents helped things along), but you do want to skin the animal. Stirring the mix also will aid in speeding up the process. If at all possible, try to keep the buckets out of direct sunlight to keep the algae from growing (I throw a tarp over my bins).

I am going to add in a caveat here since we see it so often - DO NOT ADD ANTIBACTERIAL DISH SOAP OR HYDROGEN PEROXIDE AT THIS STEP. You need bacteria to digest and break down the soft tissue. These two things make the environment hostile to bacteria causing it to take substantially longer to process. The only thing that you can add to help the process along is enzymatic detergent, which brings us to the next method.

Enzymatic Detergent Maceration - You also can add an enzymatic detergent (BIZ is one example) to the water to aid in the process. u/octane80808 has a great summary of the use of enzymatic detergents in the comments section that I have copied sections of here: "For anyone in Europe, we have enzyme-based washing powders [mod note - Biotex in Europe, powdered Biz w/out bleach in US]. I've been using it for years and I can't imagine it doing any other way. It's essentially an all-in-one method, as it dissolves the tissue, but also the fat, so it degreases at the same time. There's no need to whiten the bones afterwards, they come out perfectly clean (there is no bleach, or whitening agent present AFAIK, so it's all natural). The only downside is that any cartilage also dissolves. So for fish, birds, young animals, or small animals, you'll be spending an afternoon gluing the bones.

I also clean my skull manually during this process. Depending on how impatient I am, and how much free time I have, I take them out of the solution every day to every other day. I remove the tissue I can remove without disturbing the skull too much. And I refresh the solution. So a new spoonful of washing powder, and warm water. The latter also helps to speed up the process, as higher temperatures seem to be favourable. By refreshing the solution every day, I can usually clean a skull within one or two weeks...renewing the solution isn't necessary, it will just take a lot longer.

The washing powder is relatively cheap, and it comes in large boxes. You only have to use a teaspoon or tablespoon, depending on the size of the container/skull. So it lasts quite a long time as well..Note that the bone may feel soft after this step, especially if processing a bird or fish. It is better to allow the bone to dry before handling as this will re-harden the bone. Also, bones may turn black during this process if the water isn't changed regularly enough. Do not worry, you can treat this discoloration during the "Whitening" step."

Simmering/Cooking – this method ONLY should be used with larger animals, and can be effective when you have a carcass that is dehydrated jerky. The reason is that high heat will warp bones, and will fix the grease in the bones making step 2 (degreasing) incredibly difficult. Never use this step with birds, fish, and small mammals. To use the boiling method, you actually want your carcass to have flesh, but gutted, in order to protect the bones. Place the carcass into the boiling water and allow the water to return to a low simmer, then remove from heat. Leave the carcass in the water for only as long as it takes for the flesh to “cook” (if you are boiling for an hour, you have ruined the bones). Remove from water and the flesh should come off easily, although internal tissues (like the brain or inside the nose) will still be adhered. You can use a pressure washer or hose with a good nozzle to try and clean off the hard-to-reach areas. Be extremely cautious using a pressure washer as it will blast more fragile bone to pieces and can easily destroy a skull.

DEGREASING

You will need a degreasing agent for this step, most of the liquid dish soaps will work great here, just avoid the opaque ones or ones with strong colors (colorless and clear work great and won't dye the bones). Laundry soap often doesn’t work as well, and some will dye the bones. The exception to this is enzymatic detergent (in the US this is sold as BIZ), which works well as a degreaser. This step requires a container big enough for you to submerge the remains in. Add water and soap – how much soap is up to you and depends on the amount of grease in the bones. You will need to change the soapy water as it becomes cloudy, generally at least once a week. Continue this process until fully degreased – i.e., the water doesn’t cloud after a week. This is the longest step, and will take much longer than you think. If you see any yellowing or oily spot on the bone, then it still needs degreasing.

You can substitute acetone or ammonia for dish soap as the degreasing agent, but both have their safety issues. Ammonia is an irritant, so only use ammonia if you have a respirator. Also, be careful when emptying the liquid as household ammonia will kill vegetation.

Acetone also can be used, but you cannot dilute it with water. As a result, acetone is often more expensive that using the other two agents, and as a bonus it can melt plastic, so you will want to use a different type of container than a plastic bucket. It also dissolves nitrile and latex gloves, is flammable (no heating the liquid), and the fumes are toxic, so there is that. Also, acetone will evaporate, so the container needs to have a tight lid. If used correctly, you can treat multiple batches of bones with acetone, and acetone works faster than other methods. Lastly, acetone can’t be disposed of down the drain because of it’s toxicity and remember that bit about dissolving plastics…like your drain pipes?

WHITENING

After the bones have been degreased, you may wish to whiten the bones. This is not a necessary step, and is mainly cosmetic though it does help to sterilize the bones. You can use 3% hydrogen peroxide from the store, and it can be found in higher concentrations as hair developer, which is up to 12% hydrogen peroxide. Other options for obtaining hydrogen peroxide are from a pool supply store, though you have to be careful that it isn’t mixed with other chemicals. The important things to remember during this step is that A) hydrogen peroxide will degrade quickly when exposed to sunlight, and B) hydrogen peroxide degrades rapidly when exposed to heat, C) hydrogen peroxide will degrade faster when exposed to air. So, it works better when covered and not in direct sunlight.

Simply submerge the bone in the hydrogen peroxide until you reach the desired whiteness. If using 3% hydrogen peroxide, it isn’t necessary to dilute the liquid. Higher concentrations may require dilution as it is a powerful oxidizer.

An alternative method to submerging in hydrogen peroxide is sun bleaching. Note that this exposes the bones to the elements, and you lose a lot of control over the whitening process. It also takes considerably longer than the hydrogen peroxide approach.

NOTE: Chlorine Bleach should NEVER be used to whiten bones. Chlorine bleach degrades the bone collagen, which is the protein component of bone that holds the mineral component (hydroxyapatite) in place. This will leave the bone brittle and powdery, and the bone will continue to degrade over time. The effects are irreversible.

DRYING

I add this as a last step as this is a critical step where mistakes are often made. Bone is a porous material that contains organic components. If dried too rapidly, those organic components can shrink, or parts of the bone may dry faster than other parts. This can result in cracking, warping, and delamination of the bone. In general, let the bones dry slowly and out of the sun. Do not bake or expose it to high heat, or attempt to speed up the process. You may notice teeth cracking during the drying process. This is not uncommon, and you can glue the teeth back together after they drying process is complete.


r/bonecollecting Aug 26 '22

Official Announcement Obligatory Mod post

124 Upvotes

Well, we tried to ask politely about spamming posts with the itsaraccoon/itsalwaysaraccoon/itsapelvis comments. The downvotes also hinted that these were getting out of hand, and frankly there is no reason to put a dozen of these into a single thread. That is the very definition of spam. So, sorry folks, but there is now an automod that will remove any comments that attempt to link to one of those subs. Also note that we have created Rule 9 - no spamming. (and in case any are wondering, since the automod went live, there have been over 20 of these comments that have been removed in under 10 hrs).


r/bonecollecting 12h ago

Bone I.D. - Europe What sea creature is this?

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375 Upvotes

We found this skull on a beach in west Denmark. It was about 30-35 cm long. It didn't seem to have any holes for eyes. The teeth were small and evenly spaced. I don't even know if it's the upper or lower jaw. I'd rule out a seal, perhaps some kind of small whale? First picture is one side, second the other side, third is the neck.


r/bonecollecting 4h ago

Bone I.D. - N. America Found in a patch of woods in Minnesota near the Mississippi, what is it?

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48 Upvotes

Reminded me of a fish tooth plate but not confident on that ID and was hoping someone could identify the species.


r/bonecollecting 8h ago

Bone I.D. - N. America What is this? BC, Canada.

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70 Upvotes

What has me interested is the fact that it has canines and is this large. I should've put something in the photo for proper scale but it was easily over 12 inches long.


r/bonecollecting 11h ago

Collection my recent collection!

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62 Upvotes

my bone collection. all found by me. including a rat specimen. very proud of my collection, so… enjoy!


r/bonecollecting 4h ago

Bone I.D. - N. America Can you help me identify please found at the creek in Merriam Kansas

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15 Upvotes

r/bonecollecting 7h ago

Advice First time degreasing- how's it looking?

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27 Upvotes

It's been in acetone for a month now, I feel like it's still kind of yellow. Thinking I should leave it for another month and assess again?

But I also read guides that said it's okay to go back and forth from degreasing to whitening to see what's actually grease in the bones and what's discolorations. This skull was buried for 8 years so it had a lot of dirt stains. It has been whitened in 6% peroxide twice for 24 hours. Which got a lot of the staining out. But it still looked yellow so I thought it needed more degreasing.

What do you guys think?


r/bonecollecting 5h ago

Collection opposum degreasing wip!

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6 Upvotes

bones from two different opposums currently in the process of degreasing! I fully dried them out to see how progress is going. some cool pathology in this batch aswell ;)


r/bonecollecting 1d ago

Educational Exploring a femur with osteomyelitis

271 Upvotes

r/bonecollecting 58m ago

Collection My small bone collection

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Upvotes

The second photo is what I’ve got in the jar( much more interesting than 2 raccoon skull) I’ve got a dog bone a dear jaw, deer vertebrae I’m pretty sure, some fish bones, beaver tooth and dear pelvic bone I think and the little one is some of raccoons teeth I think


r/bonecollecting 10h ago

Advice Frog Skeleton Questions

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11 Upvotes

I found this frog skeleton in the bathroom of my workplace and would like to preserve it (likely to give to my college's paleontology lab as a comparative specimen). How do I go about cleaning such small, fragile bones?


r/bonecollecting 1d ago

Bone I.D. - N. America How old was this absolute UNIT?

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1.6k Upvotes

I found this 10-point Black-Tailed buck skull lodged in a small creek in April. It's jaw bones and the rest of the body were a few feet downstream. Since it's body was curled up, I can assume that it curled up and died of old age since animals tend to seek water sources as refuge when they're dying. And I can also assume that it died last winter because it was 99% decomposed (only a few tendons keeping the body together) and the skull got lodged in the creek, with the rest of the body drifting a few feet downstream from the winter rains. How old can we assume this buck was? The area it was found in has very few predators AND hunting is prohibited, so these bucks can live anywhere from 7-20 years old.


r/bonecollecting 10h ago

Bone I.D. - Atlantic Coast Small whale rib?

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9 Upvotes

r/bonecollecting 14h ago

Bone I.D. - N. America Bird or mammal?

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18 Upvotes

Found several of these under a great horned owl nest in the PNW. Found among many pigeon feathers, owl pellets, and mammal bones.

Looking to know whether this is from a bird or small mammal and which bone it is.


r/bonecollecting 11h ago

Bone I.D. - N. America Is this a racoon skull?

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9 Upvotes

I've had it for about 3 years, and ive always said its a raccoon skull but have never really looked further into it


r/bonecollecting 6h ago

Bone I.D. - N. America What animal is it from?

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4 Upvotes

Here is what I’m guessing is a scapula of a large marine mammal. Found on a remote beach on the Alaskan peninsula on the Bering side. It’s 15” across on both axis.


r/bonecollecting 8h ago

Bone I.D. - N. America Found at Lake Sonoma, CA

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5 Upvotes

What kind of fish(?) is this bone from?


r/bonecollecting 16h ago

Bone I.D. - N. America Skull Identification

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26 Upvotes

Found this along with another similar skull and many little bones in Aurora, CO!


r/bonecollecting 3h ago

Bone I.D. - N. America What is this bone under my front porch?

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2 Upvotes

My home was built in 1891. I was under the enclosed front porch this evening to fill holes in the stone foundation because I’ve had some mouse visitors recently. There’s debris under there, broken glass, who knows what. I’m pretty sure this is a bone but it feels somewhat light - maybe it’s just old? It was sitting right on top of some rocky-looking junk in the far corner. No chew marks that I can see but it looks like it was cut with a saw. I assume it’s from an animal that was hunted or farmed.

Scale: I’m a smaller person and my hands are about 6 inches long.


r/bonecollecting 21h ago

Bone I.D. - Europe What is it?

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53 Upvotes

Bone found in South Germany.


r/bonecollecting 17h ago

Bone I.D. - Europe Skull identification help

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19 Upvotes

Hi :) I work in a wildlife education centre in southern England and am going through our bone collection.

My colleague and I looked at the skulls and labelled what we could. Can anyone identify the numbered ones? Or correct any of the other IDs? I have included a close up of 8 (which looks like it is missing part of the front) and the underside of 3.

I suspect most will have been collected locally, but can't guarantee it. There were some other labels and bones in the box, including one label that said wild boar. Please let me know if any more photos or detail would be useful. I will be grateful for any help!


r/bonecollecting 9h ago

Bone I.D. - N. America What animal's jawbone is this is this?

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3 Upvotes

Woods in Western MA, USA. I found a lot of mandibles on line with this shape but none with molars going up the back or hooking up the front of the jaw bone. I though maybe a hog? But nothing I saw looked like this. The larger molars were as large as a nickel, smaller dime sized. Anyone know what animal this is from? It’s approximately 7” in length.


r/bonecollecting 15h ago

Bone I.D. - N. America What is this bone? My aunt says it might be a bird. Found off of Lake Champlain in Vermont.

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10 Upvotes

r/bonecollecting 2h ago

Bone I.D. - Europe Unidentified bone

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1 Upvotes